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Fluorescence immunohistology representational images depicting the colon in 56-day old female (F; n = 10) and male (M; n = 10) piglets consuming diets composed of either standard protein (SP; 18% protein) or high protein (HP; 28% protein) a . Positive staining is shown for CD45, <t>CD172a,</t> MHCII, and MIL11. Representative images showing co-staining of MIL11, CD172a, and MHCII b as well as CD45 and MHCII c are also shown. The Log 10 proportion of positive pixels for each region of interest (lamina propria) were quantified for each protein and compared between dietary protein groups d and sex e . The co-expression of CD45, CD172a, MHCII, and MIL11 proteins was determined between dietary protein groups and sex f as have the interactions between protein intake and sex g . The observed proportion of positive pixels for co-expression of CD172, MHCII, and MIL11 h and the combination of CD45, CD172a, MHCII and MIL11 i were compared against the expected counts of these combinations had the data had random distribution of expression of the proteins. Ten images were analysed per piglet and the data presented are mean ± SEM and n = 5 piglets/sex/treatment group
Mouse Anti Pig Cd172a Igg 2b, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti pig cd172a igg 2b - by Bioz Stars, 2026-09
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anti mouse igg polyclonal antibody conjugated with hrp  (Jackson Immuno)
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Fluorescence immunohistology representational images depicting the colon in 56-day old female (F; n = 10) and male (M; n = 10) piglets consuming diets composed of either standard protein (SP; 18% protein) or high protein (HP; 28% protein) a . Positive staining is shown for CD45, <t>CD172a,</t> MHCII, and MIL11. Representative images showing co-staining of MIL11, CD172a, and MHCII b as well as CD45 and MHCII c are also shown. The Log 10 proportion of positive pixels for each region of interest (lamina propria) were quantified for each protein and compared between dietary protein groups d and sex e . The co-expression of CD45, CD172a, MHCII, and MIL11 proteins was determined between dietary protein groups and sex f as have the interactions between protein intake and sex g . The observed proportion of positive pixels for co-expression of CD172, MHCII, and MIL11 h and the combination of CD45, CD172a, MHCII and MIL11 i were compared against the expected counts of these combinations had the data had random distribution of expression of the proteins. Ten images were analysed per piglet and the data presented are mean ± SEM and n = 5 piglets/sex/treatment group
Anti Mouse Igg Polyclonal Antibody Conjugated With Hrp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse igg polyclonal antibody conjugated with hrp - by Bioz Stars, 2026-09
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igg3  (Jackson Immuno)
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Fluorescence immunohistology representational images depicting the colon in 56-day old female (F; n = 10) and male (M; n = 10) piglets consuming diets composed of either standard protein (SP; 18% protein) or high protein (HP; 28% protein) a . Positive staining is shown for CD45, <t>CD172a,</t> MHCII, and MIL11. Representative images showing co-staining of MIL11, CD172a, and MHCII b as well as CD45 and MHCII c are also shown. The Log 10 proportion of positive pixels for each region of interest (lamina propria) were quantified for each protein and compared between dietary protein groups d and sex e . The co-expression of CD45, CD172a, MHCII, and MIL11 proteins was determined between dietary protein groups and sex f as have the interactions between protein intake and sex g . The observed proportion of positive pixels for co-expression of CD172, MHCII, and MIL11 h and the combination of CD45, CD172a, MHCII and MIL11 i were compared against the expected counts of these combinations had the data had random distribution of expression of the proteins. Ten images were analysed per piglet and the data presented are mean ± SEM and n = 5 piglets/sex/treatment group
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igg3 - by Bioz Stars, 2026-09
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goat anti mouse igg2b hrp  (Jackson Immuno)
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Fluorescence immunohistology representational images depicting the colon in 56-day old female (F; n = 10) and male (M; n = 10) piglets consuming diets composed of either standard protein (SP; 18% protein) or high protein (HP; 28% protein) a . Positive staining is shown for CD45, <t>CD172a,</t> MHCII, and MIL11. Representative images showing co-staining of MIL11, CD172a, and MHCII b as well as CD45 and MHCII c are also shown. The Log 10 proportion of positive pixels for each region of interest (lamina propria) were quantified for each protein and compared between dietary protein groups d and sex e . The co-expression of CD45, CD172a, MHCII, and MIL11 proteins was determined between dietary protein groups and sex f as have the interactions between protein intake and sex g . The observed proportion of positive pixels for co-expression of CD172, MHCII, and MIL11 h and the combination of CD45, CD172a, MHCII and MIL11 i were compared against the expected counts of these combinations had the data had random distribution of expression of the proteins. Ten images were analysed per piglet and the data presented are mean ± SEM and n = 5 piglets/sex/treatment group
Goat Anti Mouse Igg2b Hrp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg+2b/Peroxidase+AffiniPure+Goat+Anti-Mouse+IgG%2C+Fc%CE%B3+subclass+2b+specific/pmc12979680-110-0-5
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Fluorescence immunohistology representational images depicting the colon in 56-day old female (F; n = 10) and male (M; n = 10) piglets consuming diets composed of either standard protein (SP; 18% protein) or high protein (HP; 28% protein) a . Positive staining is shown for CD45, <t>CD172a,</t> MHCII, and MIL11. Representative images showing co-staining of MIL11, CD172a, and MHCII b as well as CD45 and MHCII c are also shown. The Log 10 proportion of positive pixels for each region of interest (lamina propria) were quantified for each protein and compared between dietary protein groups d and sex e . The co-expression of CD45, CD172a, MHCII, and MIL11 proteins was determined between dietary protein groups and sex f as have the interactions between protein intake and sex g . The observed proportion of positive pixels for co-expression of CD172, MHCII, and MIL11 h and the combination of CD45, CD172a, MHCII and MIL11 i were compared against the expected counts of these combinations had the data had random distribution of expression of the proteins. Ten images were analysed per piglet and the data presented are mean ± SEM and n = 5 piglets/sex/treatment group
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Fluorescence immunohistology representational images depicting the colon in 56-day old female (F; n = 10) and male (M; n = 10) piglets consuming diets composed of either standard protein (SP; 18% protein) or high protein (HP; 28% protein) a . Positive staining is shown for CD45, <t>CD172a,</t> MHCII, and MIL11. Representative images showing co-staining of MIL11, CD172a, and MHCII b as well as CD45 and MHCII c are also shown. The Log 10 proportion of positive pixels for each region of interest (lamina propria) were quantified for each protein and compared between dietary protein groups d and sex e . The co-expression of CD45, CD172a, MHCII, and MIL11 proteins was determined between dietary protein groups and sex f as have the interactions between protein intake and sex g . The observed proportion of positive pixels for co-expression of CD172, MHCII, and MIL11 h and the combination of CD45, CD172a, MHCII and MIL11 i were compared against the expected counts of these combinations had the data had random distribution of expression of the proteins. Ten images were analysed per piglet and the data presented are mean ± SEM and n = 5 piglets/sex/treatment group
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anti mouse igg conjugated to alkaline phosphatase - by Bioz Stars, 2026-09
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polyclonal igg  (Jackson Immuno)
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Fluorescence immunohistology representational images depicting the colon in 56-day old female (F; n = 10) and male (M; n = 10) piglets consuming diets composed of either standard protein (SP; 18% protein) or high protein (HP; 28% protein) a . Positive staining is shown for CD45, <t>CD172a,</t> MHCII, and MIL11. Representative images showing co-staining of MIL11, CD172a, and MHCII b as well as CD45 and MHCII c are also shown. The Log 10 proportion of positive pixels for each region of interest (lamina propria) were quantified for each protein and compared between dietary protein groups d and sex e . The co-expression of CD45, CD172a, MHCII, and MIL11 proteins was determined between dietary protein groups and sex f as have the interactions between protein intake and sex g . The observed proportion of positive pixels for co-expression of CD172, MHCII, and MIL11 h and the combination of CD45, CD172a, MHCII and MIL11 i were compared against the expected counts of these combinations had the data had random distribution of expression of the proteins. Ten images were analysed per piglet and the data presented are mean ± SEM and n = 5 piglets/sex/treatment group
Polyclonal Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg+2b/Cy+3+AffiniPure+Goat+Anti-Mouse+IgG+(subclasses+1%2B2a%2B2b%2B3)%2C+Fc%CE%B3+Fragment+Specific/pmc12838237-15-5-10
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Fluorescence immunohistology representational images depicting the colon in 56-day old female (F; n = 10) and male (M; n = 10) piglets consuming diets composed of either standard protein (SP; 18% protein) or high protein (HP; 28% protein) a . Positive staining is shown for CD45, CD172a, MHCII, and MIL11. Representative images showing co-staining of MIL11, CD172a, and MHCII b as well as CD45 and MHCII c are also shown. The Log 10 proportion of positive pixels for each region of interest (lamina propria) were quantified for each protein and compared between dietary protein groups d and sex e . The co-expression of CD45, CD172a, MHCII, and MIL11 proteins was determined between dietary protein groups and sex f as have the interactions between protein intake and sex g . The observed proportion of positive pixels for co-expression of CD172, MHCII, and MIL11 h and the combination of CD45, CD172a, MHCII and MIL11 i were compared against the expected counts of these combinations had the data had random distribution of expression of the proteins. Ten images were analysed per piglet and the data presented are mean ± SEM and n = 5 piglets/sex/treatment group

Journal: European Journal of Nutrition

Article Title: High-protein diets increase microbiota associated p- cresol production in the colon and reduce gut barrier function in a sex-dependent manner

doi: 10.1007/s00394-025-03885-6

Figure Lengend Snippet: Fluorescence immunohistology representational images depicting the colon in 56-day old female (F; n = 10) and male (M; n = 10) piglets consuming diets composed of either standard protein (SP; 18% protein) or high protein (HP; 28% protein) a . Positive staining is shown for CD45, CD172a, MHCII, and MIL11. Representative images showing co-staining of MIL11, CD172a, and MHCII b as well as CD45 and MHCII c are also shown. The Log 10 proportion of positive pixels for each region of interest (lamina propria) were quantified for each protein and compared between dietary protein groups d and sex e . The co-expression of CD45, CD172a, MHCII, and MIL11 proteins was determined between dietary protein groups and sex f as have the interactions between protein intake and sex g . The observed proportion of positive pixels for co-expression of CD172, MHCII, and MIL11 h and the combination of CD45, CD172a, MHCII and MIL11 i were compared against the expected counts of these combinations had the data had random distribution of expression of the proteins. Ten images were analysed per piglet and the data presented are mean ± SEM and n = 5 piglets/sex/treatment group

Article Snippet: Similarly, for the analysis of immune development in the lamina propria the following monoclonal antibodies were used: Mouse anti-pig MHC class II IgG 2a (clone MSA3, Cambridge Bioscience; 1/20 concentration), mouse anti-pig CD45 IgG 1 (clone K25 IE4, ThermoFisher Scientific, UK; 1/10 concentration), mouse anti-pig CD172a IgG 2b (clone 74-22-15, Bio-Rad, UK; 1/10 concentration), and mouse anti-pig capillary endothelial cells (IgE, clone MIL11, Bio-Rad, UK; 1/10 concentration).

Techniques: Fluorescence, Staining, Expressing